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135521 bovine serum albumin bsa gendepot cat a0100 100 vitronectin vtn n  (Thermo Fisher)


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    Thermo Fisher 135521 bovine serum albumin bsa gendepot cat a0100 100 vitronectin vtn n
    135521 Bovine Serum Albumin Bsa Gendepot Cat A0100 100 Vitronectin Vtn N, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitronectin/Bovine+Serum+Albumin/pm42269616-902-104-117
    Average 99 stars, based on 1 article reviews
    135521 bovine serum albumin bsa gendepot cat a0100 100 vitronectin vtn n - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: A South Asian Indian PRKAG2 patient-derived induced pluripotent stem cell (iPSC) line to model glycogen storage-associated hypertrophic cardiomyopathy.
    Article Snippet: Transduction of isolated PBMCs was performed after four days using CytoTune-iPS 2.0 (Thermo Fisher Scientific) Sendai virus vectors expressing human Klf4, Sox2, Oct3/4, and c-Myc, following the manufacturer’s protocol. .. The transduced PBMCs were replated onto vitronectin (Thermo Fisher Scientific) coated 12- well plates three days post transduction and the morphology was observed. ..

    Cell Culture:

    Article Title: Non-canonical enhancers control gene expression and cell fate in human pluripotent stem cells
    Article Snippet: HPSI0215i-yoch_6 was used for STARR-seq, TT-seq and CUT&Tag; HPSI0215i-yoch_6 and HPSI1113i-qolg_1 were used for luciferase assays, ATAC-seq and RNA-seq; and HPSI0215i-yoch_6 and HPSI0114i-kolf_2 were used for CRISPR. .. All cell lines were cultured in TeSR-E8 media with supplement (Stemcell Technologies, 05990) on Vitronectin (Thermo Fisher Scientific, A14700) coated surfaces at 37°C under 5% CO 2 and normal O 2 levels. ..

    Article Title: Epigenetic priming of mammalian embryonic enhancer elements coordinates developmental gene networks
    Article Snippet: .. All cell lines were cultured in TeSR-E8 media complete with supplement (Stemcell Technologies; 05990) on plates coated with vitronectin (Thermo Fisher Scientific; A14700) at 37 °C under 5% CO2 and normal O2 levels. ..

    Marker:

    Article Title: Characterization of Bruch's Membrane Formation in Human Fetal Retina and De Novo Membrane Synthesis by hPSC-Derived Retinal Pigment Epithelium.
    Article Snippet: Images were processed using the image processing software, ImageJ (Fiji). .. Brightness and contrast were adjusted equally across all timepoints for each individual marker to allow for comparison and the visualization of localization of proteins. hPSC-Derived RPE Culture The human embryonic stem cell (hESC) line H9 (WiCell, Madison, WI, USA) was maintained on vitronectin (ThermoFisher, UK) coated tissue culture plates in mTeSR Plus medium (STEMCELL Technologies, Canada). ..

    Comparison:

    Article Title: Characterization of Bruch's Membrane Formation in Human Fetal Retina and De Novo Membrane Synthesis by hPSC-Derived Retinal Pigment Epithelium.
    Article Snippet: Images were processed using the image processing software, ImageJ (Fiji). .. Brightness and contrast were adjusted equally across all timepoints for each individual marker to allow for comparison and the visualization of localization of proteins. hPSC-Derived RPE Culture The human embryonic stem cell (hESC) line H9 (WiCell, Madison, WI, USA) was maintained on vitronectin (ThermoFisher, UK) coated tissue culture plates in mTeSR Plus medium (STEMCELL Technologies, Canada). ..

    Saline:

    Article Title: Donor Age Impairs Vasculogenic Potential of hiPSC-Derived Endothelial Progenitors
    Article Snippet: All hiPSC lines were cultured on vitronectin-coated plates (Thermo Fisher) and maintained in Essential 8 (E8) medium (Thermo Fisher). .. Plates were coated with vitronectin according to the manufacturer’s protocol, using a 0.5 μg/mL solution prepared in Dulbecco’s phosphate-buffered saline (DPBS) and incubated at 37°C for 1 hour prior to hiPSC seeding. hiPSCs were colony passaged every 3–4 days at approximately 70–80% confluency using 0.5 mM Ethylenediaminetetraacetic acid (EDTA) in DPBS (Thermo Fisher). ..

    Incubation:

    Article Title: Donor Age Impairs Vasculogenic Potential of hiPSC-Derived Endothelial Progenitors
    Article Snippet: All hiPSC lines were cultured on vitronectin-coated plates (Thermo Fisher) and maintained in Essential 8 (E8) medium (Thermo Fisher). .. Plates were coated with vitronectin according to the manufacturer’s protocol, using a 0.5 μg/mL solution prepared in Dulbecco’s phosphate-buffered saline (DPBS) and incubated at 37°C for 1 hour prior to hiPSC seeding. hiPSCs were colony passaged every 3–4 days at approximately 70–80% confluency using 0.5 mM Ethylenediaminetetraacetic acid (EDTA) in DPBS (Thermo Fisher). ..

    other:

    Article Title: Characterization of Bruch's Membrane Formation in Human Fetal Retina and De Novo Membrane Synthesis by hPSC-Derived Retinal Pigment Epithelium
    Article Snippet: The human embryonic stem cell (hESC) line H9 (WiCell, Madison, WI, USA) was maintained on vitronectin (ThermoFisher, UK) coated tissue culture plates in mTeSR Plus medium (STEMCELL Technologies, Canada).



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    Image Search Results


    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Journal: bioRxiv

    Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

    doi: 10.64898/2026.03.19.712761

    Figure Lengend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Article Snippet: Antibodies against LC3B (Sigma, L8918-200UL), actin (Proteintech, 66009-1-Ig), PLIN2 (Proteintech, 15294-1-AP), fibronectin (Invitrogen, PA5-29578), clusterin (R&D systems, AF2747), vitronectin (R&D Systems, MAB38751), and p62 (Abnova, H00008878-M01; CST, 5114), Lamp2a (Abcam, ab125068), Lamp2b (Abcam, ab13524), Iba1 (Fujifilm Wako, 019-19741), Laminin (Sigma, L9393) and ApoE (Abcam, ab183596) were used in this study.

    Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot